Voltage clamp experiments on internally perfused giant axons.
نویسندگان
چکیده
منابع مشابه
Voltage Clamp Studies on Internally Perfused Axons
Probably the most striking and surprising observation in internally perfused axons is that full-sized action potentials can be obtained in the face of a very low resting potential when the ionic strength is low In all experiments at normal ionic strength known to the author, the explosive all-or-none responsiveness was lost when the internal and/or external monovalent cations were arranged to g...
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An improved method for internally perfusing the Myxicola giant axon based on removing the axoplasm by dispersing it in KCl-KF salt solutions is described. Proteolytic enzymes are not introduced. With this improved method perfused preparations show long-term stability of their electrical properties and the ability to generate action potentials for many hours. Mean initial values for resting memb...
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Squid giant axons were internally perfused with tetrodotoxin and procaine, and excitability and electrical properties were studied by means of current-clamp and sucrose-gap voltage-clamp methods. Internally perfused tetrodotoxin was virtually without effect on the resting potential, the action potential, the early transient membrane ionic current, and the late steady-state membrane ionic curren...
متن کاملIonic Selectivity in Perfused Giant Axons
Information about the ionic selectivity of the sodium-carrying system of the active nerve membrane has been obtained by voltage clamp experiments on internally perfused giant axons of Loligo forbesi. These experiments were carried out in the Laboratory of the Marine Biological Association in Plymouth during the winter 1963-64. Uncleaned axons were perfused by the method of Baker, Hodgkin, and S...
متن کاملSodium Movements in Perfused Squid Giant Axons
Sodium movements in internally perfused giant axons from the squid Dosidicus gigas were studied with varying internal sodium concentrations and with fluoride as the internal anion. It was found that as the internal concentration of sodium was increased from 2 to 200 mM the resting sodium efflux increased from 0.09 to 34.0 pmoles/cm(2)sec and the average resting sodium influx increased from 42.9...
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ژورنال
عنوان ژورنال: The Journal of Physiology
سال: 1965
ISSN: 0022-3751
DOI: 10.1113/jphysiol.1965.sp007732